METHODS, SYSTEMS AND COMPOSITIONS REGARDING MULTIPLEX CONSTRUCTION PROTEIN AMINO-ACID SUBSTITUTIONS AND IDENTIFICATION OF SEQUENCE-ACTIVITY RELATIONSHIPS, TO PROVIDE GENE REPLACEMENT SUCH AS WITH TAGGED MUTANT GENES, SUCH AS VIA EFFICIENT HOMOLOGOUS RECOMBINATION

• Main Author: WARNER JOSEPH R
• Format: Patent
• Language: English
• Published: 01.05.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A method is provided to construct libraries of nucleic acids that comprise a plurality of mutations, each of which may be identified with particularity, such as following a selection or screening.