METHOD FOR TRANSFORMING STRAMENOPILES

Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifica...

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Main Authors HAYASHI MASAHIRO, OKITA YUJI, SUGIMOTO SHINICHI, IZUMIDA HITOSHI, ITO MAKOTO, MATSUDA TAKANORI, NAGANO NAOKI, TAOKA YOSUKE, SAKAGUCHI KEISHI, HONDA DAISUKE, KOBAYASHI TAKUMI
Format Patent
LanguageEnglish
Published 20.12.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase ( 5 desaturase gene, 12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
Bibliography:Application Number: US201013497894