MICROFLUIDIC DEVICE AND METHODS OF USING SAME

• Main Authors: LUCERO MICHAEL, MIYASHITA-LIN EMILY, WIENECKE ANJA, MANGER IAN D, YI YONG, UNGER MARC, FACER GEOFFREY
• Format: Patent
• Language: English
• Published: 04.01.2007
• Subjects: ACTUATING-FLOATS; APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; BLASTING; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COCKS; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DEVICES FOR VENTING OR AERATING; ENGINEERING ELEMENTS AND UNITS; ENZYMOLOGY; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVEFUNCTIONING OF MACHINES OR INSTALLATIONS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HEATING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; LIGHTING; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MECHANICAL ENGINEERING; METALLURGY; MICROBIOLOGY; MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; POSITIVE DISPLACEMENT MACHINES FOR LIQUIDS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PUMPS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS; SPIRITS; TAPS; TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION; TECHNICAL SUBJECTS COVERED BY FORMER USPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TESTING; THEIR RELEVANT APPARATUS; THERMAL INSULATION IN GENERAL; TRANSPORTING; VALVES; VINEGAR; WEAPONS; WINE

A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.