Method and apparatus for multiple labeling detection and evaluation of a plurality of particles

The invention relates to a transmission electron microscope equipped with a 2k-2k pixel area Slow Scan Cooled Charge Coupled Device Camera connected to an image processing software for generating an image of a sample. A segmentation of gold particles in the sample is achieved by the separation from...

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Bibliographic Details
Main Authors TROESTER HELMUT, TRENDELENBURG MICHAEL, MONTEIRO-LEAL LUIZ H, SPRING HERBERT, CAMPANATI-ARAUJO LORAINE
Format Patent
LanguageEnglish
Published 21.09.2006
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CALCULATING
CHEMISTRY
COMPUTING
COUNTING
ENZYMOLOGY
HANDLING RECORD CARRIERS
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
OPTICS
PHYSICS
PRESENTATION OF DATA
RECOGNITION OF DATA
RECORD CARRIERS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:The invention relates to a transmission electron microscope equipped with a 2k-2k pixel area Slow Scan Cooled Charge Coupled Device Camera connected to an image processing software for generating an image of a sample. A segmentation of gold particles in the sample is achieved by the separation from specimen structure and background noise. An identification and classification of particle types is carried out according to the shape and size of the detected particles or particle pairs and finally, the gold particle distribution is visualized by the generation of false color overlay images as well as the indication of the numbers in the image.
Bibliography:Application Number: US20030522390