Discrete zoned microporous nylon coated glass platform for use in microwell plates and methods of making and using same

• Main Authors: MEYERING MARK T, ARNOLD TODD E, SPEARIN AARON, SOLOMON KEITH, WESNER KEITH, MEIER PETER M, HE ZHIJUN
• Format: Patent
• Language: English
• Published: 25.05.2006
• Subjects: PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; TRANSPORTING

The present disclosure relates to processes and methods for producing a hydrophobic zone boundary that surrounds a hydrophilic porous material layer mounted on a substrate, the hydrophilic porous material layer containing tortuous channels and pores such that the fluid contained within one hydrophilic layer region does not cross the hydrophobic zone boundary and the articles formed thereby and, more particularly, to processes and methods for producing a hydrophobic zone boundary that separates adjacent regions of a hydrophilic porous material layer mounted on a substrate, the hydrophilic porous material layer containing tortuous channels and pores mounted on a substrate such that a uniform hydrophobic zone boundary layer in the z-direction is formed in the hydrophilic porous material or the removal of the hydrophilic porous material layer from the substrate to form a hydrophilic porous material zone on the substrate, the so formed hydrophilic porous material zone having a predetermined geometric shape such that the combination produced thereby is useful in microarray applications and other applications. Methods of making and using discrete zoned microporous nylon coated glass plateforms for use with microwell plates are also disclosed.