Method for producing liposomal formulations of active ingredients

• Main Authors: UNGER CLEMENS, MASSING ULRICH, MOOG REGINA
• Format: Patent
• Language: English
• Published: 10.02.2005
• Edition: 7
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

The invention relates to a method for producing liposomes which comprise as active ingredient at least one physiologically active or/and diagnostic compound, by production of a mixture of membrane-forming amphiphiles, dispersion of the mixture in water and high pressure homogenization of the dispersion until the liposomes are formed, wherein (a) the mixture is adjusted to result in a liposome preparation containing at least 20% by weight lipids, (b) the active ingredient is added to this liposome preparation and (c) the active ingredient-containing mixture is incubated with mechanical agitation until at least 10% of the liposomes have a content of the active ingredient, the temperature being controlled so that negligible phospholipid hydrolysis occurs, but diffusion is increased. The invention also relates to the liposome preparation produced by this method and to the use of this liposome preparation as diagnostic or therapeutic composition, which, where appropriate, contains further excipients, carriers or/and stabilizers.