Detection of RNA

• Main Authors: CURTIS MICHELLE L, OLSON SARAH M, OLSON-MUNOZ MARILYN C, MA WUPO, KWIATKOWSKI ROBERT W, ALLAWI HATIM, HALL JEFF G, LUKOWIAK ANDREW A, BARTHOLOMAY CHRISTIAN TOR, EIS PEGGY S, SCHAEFER JAMES J, CHEHAK LUANNE, SKRZYPCZYNSKI ZBIGNIEW, TAKOVA TSETSKA Y, NERI BURCE P, VEDVIK KEVIN L, IP HON S, KAISER MICHAEL, LYAMICHEV VICTOR, LYAMICHEV NATALIE
• Format: Patent
• Language: English
• Published: 05.06.2003
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ELECTRIC COMMUNICATION TECHNIQUE; ELECTRICITY; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHICCOMMUNICATION; VINEGAR; WINE

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.