Cell-preservation liquid and method of preserving cells by using the liquid

• Main Authors: BAMBA KENZO, KUROIWA YASUYUKI, SEKINE TERUAKI
• Format: Patent
• Language: English
• Published: 16.01.2003
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; TRAPPING

This invention relates to a cell-preservation liquid having an excellent performance to preserve cells without serum nor culture solution, and a cell-preserving method using the cell-preservation liquid. The cell-preservation liquid containing neither serum nor culture solution comprises a main agent prepared by admixing albumin contained in phosphate buffer solution so far applied to preserve germ cells with dimethyl sulfoxide used as an additive to a conventional serum-contained preservation liquid. Further, the cell-preservation liquid optionally contains an auxiliary agent such as phosphate ion, divalent metal ions and monosaccharides according to need. Therefore, the preservation liquid has no need of containing serum and culture solution, consequently to be free from danger of denaturing the cells and causing the cells to be contaminated with unknown viruses. Moreover, the present invention can provide the cell-preservation liquid comprising definite components has highly stable qualities and show an excellent performance to preserve cells, and an extremely useful method for preserving cells using the cell-preservation liquid.