Methods for coexpression of more than one gene in eukaryotic cells
A primary object of this invention is to provide a method which will enable to coexpress simultaneously two (or more) desired genes in plant, animal or yeast cells, in transgenic plants and animals, or in vitro, in plant cell-derived or animal cell-derived translation systems. This objection is to b...
Saved in:
Main Authors | , , , , , , |
---|---|
Format | Patent |
Language | English |
Published |
21.03.2002
|
Edition | 7 |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | A primary object of this invention is to provide a method which will enable to coexpress simultaneously two (or more) desired genes in plant, animal or yeast cells, in transgenic plants and animals, or in vitro, in plant cell-derived or animal cell-derived translation systems. This objection is to be accomplished by utilizing sequence elements derived from RNAs of a tobamovirus upstream of MP gene or CP gene termed here as IRESmp and IREScp , respectively. The method of this invention involves the construction of a recombinant nucleic acid sequence which comprises a specific transcriptional promoter, a first gene expressible in eukaryotic cells linked to said transcriptional promoter, IRESmp or IREScp located 3' to the first gene and a second gene expressible in eukaryotic cells, located 3' to IRES sequence such that the second gene is placed under the transcriptional control of IRES sequence originated from tobamovirus genome. The primary chimeric RNA transcript in positive sense polarity is produced by the transformed cells from the said promoter. The expression of the first gene occurs by direct translation whereas the translation of the 5'-distal gene(s) of bicistronic (or polycistronic) mRNA will be promoted by IRESmp or IREScp. |
---|---|
AbstractList | A primary object of this invention is to provide a method which will enable to coexpress simultaneously two (or more) desired genes in plant, animal or yeast cells, in transgenic plants and animals, or in vitro, in plant cell-derived or animal cell-derived translation systems. This objection is to be accomplished by utilizing sequence elements derived from RNAs of a tobamovirus upstream of MP gene or CP gene termed here as IRESmp and IREScp , respectively. The method of this invention involves the construction of a recombinant nucleic acid sequence which comprises a specific transcriptional promoter, a first gene expressible in eukaryotic cells linked to said transcriptional promoter, IRESmp or IREScp located 3' to the first gene and a second gene expressible in eukaryotic cells, located 3' to IRES sequence such that the second gene is placed under the transcriptional control of IRES sequence originated from tobamovirus genome. The primary chimeric RNA transcript in positive sense polarity is produced by the transformed cells from the said promoter. The expression of the first gene occurs by direct translation whereas the translation of the 5'-distal gene(s) of bicistronic (or polycistronic) mRNA will be promoted by IRESmp or IREScp. |
Author | DOROKHOV YURII SKULACHEV MAXIM KORPELA TIMO ATABEKOV JOSEPH KARPOVA OLGA IVANOV PETER RODIONOVA NINA |
Author_xml | – fullname: DOROKHOV YURII – fullname: IVANOV PETER – fullname: KORPELA TIMO – fullname: KARPOVA OLGA – fullname: SKULACHEV MAXIM – fullname: RODIONOVA NINA – fullname: ATABEKOV JOSEPH |
BookMark | eNrjYmDJy89L5WRw8k0tychPKVZIyy9SSM5PrSgoSi0uzszPU8hPU8jNL0pVKMlIBHLyUhXSU4FEZp5Caml2YlFlfklmskJyak5OMQ8Da1piTnEqL5TmZlB2cw1x9tBNLciPTy0uSEwGaiyJDw02MjAwMjA2sTA0cTQ0Jk4VABPpNIY |
ContentType | Patent |
DBID | EVB |
DatabaseName | esp@cenet |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: EVB name: esp@cenet url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP sourceTypes: Open Access Repository |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Chemistry Sciences |
Edition | 7 |
ExternalDocumentID | US2002034814A1 |
GroupedDBID | EVB |
ID | FETCH-epo_espacenet_US2002034814A13 |
IEDL.DBID | EVB |
IngestDate | Fri Jul 19 15:19:20 EDT 2024 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-epo_espacenet_US2002034814A13 |
Notes | Application Number: US20010911732 |
OpenAccessLink | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20020321&DB=EPODOC&CC=US&NR=2002034814A1 |
ParticipantIDs | epo_espacenet_US2002034814A1 |
PublicationCentury | 2000 |
PublicationDate | 20020321 |
PublicationDateYYYYMMDD | 2002-03-21 |
PublicationDate_xml | – month: 03 year: 2002 text: 20020321 day: 21 |
PublicationDecade | 2000 |
PublicationYear | 2002 |
RelatedCompanies | DOROKHOV YURII SKULACHEV MAXIM KORPELA TIMO ATABEKOV JOSEPH KARPOVA OLGA IVANOV PETER RODIONOVA NINA |
RelatedCompanies_xml | – name: ATABEKOV JOSEPH – name: KORPELA TIMO – name: SKULACHEV MAXIM – name: IVANOV PETER – name: RODIONOVA NINA – name: KARPOVA OLGA – name: DOROKHOV YURII |
Score | 2.5538006 |
Snippet | A primary object of this invention is to provide a method which will enable to coexpress simultaneously two (or more) desired genes in plant, animal or yeast... |
SourceID | epo |
SourceType | Open Access Repository |
SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
Title | Methods for coexpression of more than one gene in eukaryotic cells |
URI | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20020321&DB=EPODOC&locale=&CC=US&NR=2002034814A1 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dS8MwED_GFPVNp-LHlIDSt2LTdv14KGK_GEK34VbZ21jbFIbSjrVD_e-9hFb3tLeQgyMJudxH7u4H8JgpRkZNZssM1aWsa6kpJ7mhyqmdW2a-pIkusAGjkTGM9df5YN6Bz7YWRvQJ_RLNEVGiUpT3WrzX6_8gli9yK6unZIVT5XM4c3yp9Y45HjiVfNcJJmN_7Eme58RTafTW0HSL6i_oKx1wQ5p32g_eXV6Xst5VKuEpHE6QX1GfQYcVPTj2Wuy1HhxFzZc3Dhvpq87BjQTcc0XQ0CRpyb6bJNaClDnhCbOEx8FJWTCC14KRVUHY9mO5-SnxdhAeoq8u4CEMZt5QxsUs_va-iKe7K9cuoVsglysgSTawmUXRH7E5jJhiq0xPFC3LbCNVUqZcQ38fp5v95Fs4EbAniiartA_derNld6h96-ReHNovzBSIAQ |
link.rule.ids | 230,309,786,891,25594,76904 |
linkProvider | European Patent Office |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dS8MwED_GFOebTsWPqQGlb8V27frxMMS2K1XXbbhW9jbWNoWhtGPtUP97L6HVPe0tJHBcwl0ud7m7H8B9ImmJrFNTpGguRVWJdTFKta4Ym6mhpws5Ujk2oD_SvFB9mfVmDfisa2F4n9Av3hwRNSpGfS_5fb36D2I5PLeyeIiWOJU_ukHfEWrvmOGBy4Jj9QeTsTO2Bdvuh1Nh9FatqYasPqGvtKejU8g67Q_eLVaXsto2Ku4R7E-QXlYeQ4NmbWjZNfZaGw786ssbh5X2FSdg-RzuuSD40CRxTr-rJNaM5ClhCbOExcFJnlGCYkHJMiN087FY_-QoHYSF6ItTuHMHge2JyMz8b-_zcLrNuXIGzQypnAOJkp5JDRn9EZPBiElml6qRpCSJqcVSTKUL6OyidLl7-RZaXuAP58Pn0esVHHIIFEkRu3IHmuV6Q6_REpfRDT_AX071iuw |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=Methods+for+coexpression+of+more+than+one+gene+in+eukaryotic+cells&rft.inventor=DOROKHOV+YURII&rft.inventor=IVANOV+PETER&rft.inventor=KORPELA+TIMO&rft.inventor=KARPOVA+OLGA&rft.inventor=SKULACHEV+MAXIM&rft.inventor=RODIONOVA+NINA&rft.inventor=ATABEKOV+JOSEPH&rft.date=2002-03-21&rft.externalDBID=A1&rft.externalDocID=US2002034814A1 |