P0 gene silencing constructs and use

• Main Authors: Brault, Véronique, Graff, Véronique, Lefebvre, Marc, Gilmer, David, Weyens, Guy, Klein, Elodie
• Format: Patent
• Language: English
• Published: 26.05.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention include a recombinant BMYV P0 viral nucleotide sequence, which when transcribed in a cell, is capable of forming a double stranded self-complementary RNA sequence. The invention is related to a method for conveying viral resistance or tolerance to one or more virus(es), in particular to beet mild yellowing virus (BMYV) and to beet necrotic yellow vein virus (BNYVV) or to BMYV alone in a plant, in particular in a sugar beet plant. Furthermore, the present invention relates to the virus-resistant or -tolerant plant obtained according to this method, as well as to seeds and progeny derived therefrom.