Massive parallel method for decoding DNA and RNA
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotid...
Saved in:
Main Authors | , , , |
---|---|
Format | Patent |
Language | English |
Published |
10.09.2019
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the -OH group at the 3′-position of the deoxyribose. |
---|---|
AbstractList | This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the -OH group at the 3′-position of the deoxyribose. |
Author | Itagaki, Yasuhiro Edwards, John Robert Li, Zengmin Ju, Jingyue |
Author_xml | – fullname: Li, Zengmin – fullname: Edwards, John Robert – fullname: Itagaki, Yasuhiro – fullname: Ju, Jingyue |
BookMark | eNrjYmDJy89L5WQw8E0sLs4sS1UoSCxKzMlJzVHITS3JyE9RSMsvUkhJTc5PycxLV3Dxc1RIzEtRCPJz5GFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8aHBhgYmBuYmphZORsbEqAEA5wkr9A |
ContentType | Patent |
DBID | EVB |
DatabaseName | esp@cenet |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: EVB name: esp@cenet url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP sourceTypes: Open Access Repository |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Chemistry Sciences Physics |
ExternalDocumentID | US10407458B2 |
GroupedDBID | EVB |
ID | FETCH-epo_espacenet_US10407458B23 |
IEDL.DBID | EVB |
IngestDate | Fri Sep 27 05:20:32 EDT 2024 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-epo_espacenet_US10407458B23 |
Notes | Application Number: US201816149098 |
OpenAccessLink | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190910&DB=EPODOC&CC=US&NR=10407458B2 |
ParticipantIDs | epo_espacenet_US10407458B2 |
PublicationCentury | 2000 |
PublicationDate | 20190910 |
PublicationDateYYYYMMDD | 2019-09-10 |
PublicationDate_xml | – month: 09 year: 2019 text: 20190910 day: 10 |
PublicationDecade | 2010 |
PublicationYear | 2019 |
RelatedCompanies | The Trustees of Columbia University in the City of New York |
RelatedCompanies_xml | – name: The Trustees of Columbia University in the City of New York |
Score | 3.2299125 |
Snippet | This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide... |
SourceID | epo |
SourceType | Open Access Repository |
SubjectTerms | APPARATUS THEREFOR BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY GENERAL METHODS OF ORGANIC CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING VINEGAR WINE |
Title | Massive parallel method for decoding DNA and RNA |
URI | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190910&DB=EPODOC&locale=&CC=US&NR=10407458B2 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LS8NAEB5Kfd40KlofrCC5BfN-HII0L4qQtLSN9FaSzQYqkhYT8e87G1PrRa-zsOwOzHwzuzPfADzo1ClthdqSplpM0jNKJYQRRXIs1aCcbUQxeXNynJijVH9eGIsevG57YVqe0M-WHBEtiqK9N62_3uwesYK2trJ-zFcoWj9FczcQu-wY0Q3hTww8N5yMg7Ev-r6bzsRkyofcIlgatofueo-H0ZxnP3zxeFfK5jekRCewP8HdquYUeqwS4MjfTl4T4DDuPrwFOGgrNGmNws4K6zOQY4x40UsRztuNef8b-Z4DTTAAJQXmkxyPSJAMSVYVZJoMz-E-Cuf-SMJDLH9uvExnu_NqF9Cv1hW7BKKpsmWVtFQ0kw_YZY6Ta0wv1EyxzYJZ9hUM_t5n8N_iNRxz7fFCCEW-gX7z_sFuEW2b_K5V0xfjYYFH |
link.rule.ids | 230,309,786,891,25594,76903 |
linkProvider | European Patent Office |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT8JAEJ4QfOBNqwbxtSamt8a-H4fG0BaCSguB1nAjsF0SjSlEavz7zq5FvOh1N5nMbjLzzbc7D4Bbk3oLV6OuYugOU8wZpQrCiKZ4jm5R3m1Es3lxcpzYvcx8nFiTGrxuamFEn9BP0RwRLYqivZfCX6-2j1iRyK1c381fcGl53039SK7YMaIbwp8cBX5nOIgGoRyGfjaWkxEfcotgabkBuusdBymhoErPAa9KWf2GlO4h7A5RWlEeQY0VEjTCzeQ1Cfbj6sNbgj2RoUnXuFhZ4foY1BgjXvRShPftRt7_Rr7nQBMMQEmOfJLjEYmSNpkVORkl7RO46XbSsKegEtOfE0-z8VZf4xTqxbJgTSCGrjrOgi40w-YDdpnnzQ1m5vpMc-2cOe4ZtP6W0_pv8xoavTTuT_sPydM5HPCb5EkRmnoB9fL9g10i8pbzK3FlXxOyhDE |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=Massive+parallel+method+for+decoding+DNA+and+RNA&rft.inventor=Li%2C+Zengmin&rft.inventor=Edwards%2C+John+Robert&rft.inventor=Itagaki%2C+Yasuhiro&rft.inventor=Ju%2C+Jingyue&rft.date=2019-09-10&rft.externalDBID=B2&rft.externalDocID=US10407458B2 |