Method for amplifying target gene with specific GC content

The present invention provides a method for amplifying at least one target gene having a GC content of 60% or greater in a reaction solution, wherein: the method includes a step for amplifying a base sequence specific to a target gene using a primer set composed of a first primer and a second primer...

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Bibliographic Details
Main Authors MIYATAKE, YUYA, TATEDA, KAZUHIRO
Format Patent
LanguageChinese
English
Published 01.01.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a method for amplifying at least one target gene having a GC content of 60% or greater in a reaction solution, wherein: the method includes a step for amplifying a base sequence specific to a target gene using a primer set composed of a first primer and a second primer for amplifying the target gene; one of the primers in the primer set is added to the reaction solution in a higher molar ratio than the other primer; and neither of the primers is a hot-start primer that includes an inactivating chemical modification restored by the action of an activating enzyme.
Bibliography:Application Number: TW202211111170