METHOD FOR RESTORING BIOSYNTHESIS ABILITY OF PEST MICROBE CAPSULE ANTIGEN

• Main Authors: ZAJTSEV A.A, TSAREVA N.S, EVCHENKO JU.M, SHCHEDRIN V.I
• Format: Patent
• Language: English
• Published: 10.09.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

medicine. SUBSTANCE: method involves subcutaneously injecting 0.5-0.7 ml of culture suspension under test in physiological salt solution in 103-105 cell mln/ml concentration. The culture is previously mixed to hen egg yolk in 1:0.5-1:0.7 proportion. 1.5-2.0 ml of citrated sheep blood and 5-7 mg of cyclophosphane are added to the produced mixture. Pathologic material is taken from the place the culture under test has been introduced in 46- 48 h. The sample is cultured on Hottinger agar of pH 7.2 with 1% hemolyzed blood at 37 C during 48 h. Capsule antigen biosynthesis is studied in serologic reactions. EFFECT: optimum conditions for restore capsule antigen production. 1 tbl