METHODS OF PRODUCING BIOCONJUGATES OF E. COLI O-ANTIGEN POLYSACCHARIDES, COMPOSITIONS THEREOF, AND METHODS OF USE THEREOF

• Main Authors: GEURTSEN, JEROEN, POOLMAN, JAN, THEUNIS, BURGHOUT, PIETER, JAN, FAE, KELLEN, CRISTHINA, MALLY, MANUELA, IBARRA YON, PATRICIA, KEMMLER, STEFAN, JOCHEN, KOWARIK, MICHAEL, THOMAS, GAMBILLARA, FONCK, VERONICA, WEERDENBURG, EVELINE, MARLEEN, CARRANZA SANDMEIER, MARIA, PAULA, BRAUN, MARTIN, EDWARD, ABBANAT, DARREN, ROBERT
• Format: Patent
• Language: English; Serbian
• Published: 29.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysacch...