Growth hormone polypeptides and methods of making and using same

596787 Disclosed is a fusion protein, comprising a growth hormone (GH) sequence selected from human growth hormone of SEQ ID NO: 1 and an agonist thereof that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the amino acid sequence FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQ...

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Bibliographic Details
Main Authors GEETHING NATHAN, WANG CHIA-WEI, CLELAND JEFFREY L, STEMMER WILLEM P, SILVERMAN JOSHUA, SPINK BENJAMIN, SCHELLENBERGER VOLKER
Format Patent
LanguageEnglish
Published 28.03.2014
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:596787 Disclosed is a fusion protein, comprising a growth hormone (GH) sequence selected from human growth hormone of SEQ ID NO: 1 and an agonist thereof that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the amino acid sequence FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF, wherein said GH is linked to an extended recombinant polypeptide (XTEN) of at least about 200 amino acid residues, wherein the XTEN is characterized in that: (a) the XTEN sequence comprises at least about 200 contiguous amino acids that exhibits at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to a comparable length of an amino acid sequence selected from sequences such as MAEPAGSPTSTEEGTPGSGTASSSPGSSTPSGATGSPGASPGTSSTGS and MAEPAGSPTSTEEGASPGTSSTGSPGSSTPSGATGSPGSSTPSGATGS (b) the XTEN sequence lacks a predicted T-cell epitope when analyzed by TEPITOPE algorithm, wherein the TEPITOPE algorithm prediction for epitopes within the XTEN sequence is based on a score of -9 or greater; (c) it has a subsequence score of less than 10; and (d) the sum of glycine (G), alanine (A), serine (S), threonine (T), glutamate (E) and proline (P) residues constitutes more than about 90% of the total amino acid residues of the XTEN.
Bibliography:Application Number: NZ20100596787