INTEGRATED NUCLEIC ACID ANALYSIS

• Main Authors: SELDEN, RICHARD, F, KELLOGG, GREGORY, JOHN, WRIGHT, JOHN, A, LAM, HEUNG, CHUAN, BOGDANOV, VALERY, LEONIDOVICH, ULRICH, HANS, THOMANN, TAN, EUGENE
• Format: Patent
• Language: English
• Published: 24.02.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; TRANSPORTING; VINEGAR; WINE

Disclosed is an integrated biochip system comprising: (a) a biochip comprising a plurality microfluidic systems, wherein each microfluidic system comprises a first reaction chamber in microfluidic communication with a separation chamber, wherein the first reaction chamber is adapted for (i) nucleic acid extraction; (ii) nucleic acid purification; (iii) pre-nucleic acid amplification cleanup; (iv) nucleic acid amplification; (v) post- nucleic acid amplification cleanup; (vi) pre- nucleic acid sequencing cleanup; (vii) nucleic acid sequencing; (viii) post- nucleic acid sequencing cleanup; (ix) reverse transcription; (x) pre-reverse transcription cleanup; (xi) post-reverse transcription cleanup; (xii) nucleic acid ligation; (xiii) nucleic acid hybridization; or (xiv) quantification; and the separation chamber comprises a detection position; and (b) a separation and detection system comprising, (i) a separation element for simultaneously separating a plurality of target analytes in the separation chamber; (ii) one or more light sources positioned to illuminate the detection positions on the biochip; (iii) a mirror to scan said one or more light sources sequentially between said detection positions; (iv) one or a plurality of first optical elements positioned for collecting and directing light emanating from the detection positions; and (v) a light detector positioned to accept light directed from the one or plurality of first optical elements, wherein the light detector comprises a wavelength dispersive element to disperse the light from the one or plurality of first optical elements according to light wavelength into at least 6 wavelength components and, the wavelength dispersive element is positioned to provide at least a portion of the dispersed at least 6 wavelength components to at least 6 detection elements, Wherein each of the detection elements is in communication with a first control element for simultaneously collecting detection information from each of the detection elements; and wherein said light detector detects fluorescence from at least 6 dyes labeled to one or more biological molecules, each dye having a unique peak wavelength.