Device and Method of preparation of pharmaceutical grade plasmid DNA

• Main Authors: BLANCHE, FRANCIS, MAESTRALI, NICOLAS, COUDER, MICHEL, GUILLEMIN, THIERRY, GAILLAC, DAVID
• Format: Patent
• Language: English
• Published: 31.07.2009
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A device and a method of using it for lysing cells and isolating plasmid DNA from cells are disclosed. The device includes a means for generating turbulent flow to rapidly mix a cell suspension with a solution that lyses cells, and a means for generating laminar flow to permit incubating the mixture...