Device and Method of preparation of pharmaceutical grade plasmid DNA

A device and a method of using it for lysing cells and isolating plasmid DNA from cells are disclosed. The device includes a means for generating turbulent flow to rapidly mix a cell suspension with a solution that lyses cells, and a means for generating laminar flow to permit incubating the mixture...

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Bibliographic Details
Main Authors BLANCHE, FRANCIS, MAESTRALI, NICOLAS, COUDER, MICHEL, GUILLEMIN, THIERRY, GAILLAC, DAVID
Format Patent
LanguageEnglish
Published 31.07.2009
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A device and a method of using it for lysing cells and isolating plasmid DNA from cells are disclosed. The device includes a means for generating turbulent flow to rapidly mix a cell suspension with a solution that lyses cells, and a means for generating laminar flow to permit incubating the mixture formed without substantial agitation, wherein the mixture flows from the means for generating turbulent flow into the means for generating laminar flow.
Bibliography:Application Number: NZ20040545449