PRIMERS, PROBE, KIT AND METHOD FOR FLUORESCENCE QUANTITATIVE PCR DETECTION OF PHENACOCCUS SOLANI FERRIS

• Main Authors: WENYUAN FANG, ZHIYI WU, YING WU, PENGCHENG CHEN, ZHIHAO DANG, FAN CHENG, HONGWEI TIAN, FANG HUANG, HUIJI TANG
• Format: Patent
• Language: English
• Published: 05.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention belongs to the technical field of molecular biology, and particularly 5 relates to primers, probe, kit and method for real-time fluorescence quantitative PCR (qPCR). detection of Phenacoccus solani Ferris, wherein the method comprises the following steps: DNA extraction: respectively extracting DNA of adult insects of six species of Pseudococcidae by a kit, putting adult insects in a 1.5ml centrifuge tube, and adding liquid nitrogen to grind into powder. The present invention has the advantages that the identification of Phenacoccus solani Ferris, 10 and other insects of Phenacoccus sp. and Dysmicoccus sp. is rapidly and accurately realized, the confusion possibly caused by morphological identification is avoided, the time consumed for qPCR is greatly shortened, a detection result is obtained in only 1h; and has the advantages of high specificity and high sensitivity. The minimum DNA concentration of detection can reach 100fg/uL, the result can be directly observed, the pollution caused in the operation process can 15 be effectively avoided, and Phenacoccus solani can be quickly and accurately detected by means of the primers, probe and kit for qPCR detection.