PROCESS MAKING OF ANTI T-2 TOXIN MONOCLONAL ANTIBODY AND METHOD ANALYSIS A T-2 TOXIN

A method for the production of anti T-2 toxin monoclonal antibody or its reactive fragment comprises (a) in vitro immunization of B-lymphocyte of rodents by antigen using T-2 toxin as hapten, (b) cell fusion of the immunized lymphocyte and myeloma cell to form hybridoma, (c) selecting for clones pro...

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Main Authors KIM JION, OH SANG-GON, CHONG YUN-SU, YUN HUI-JU, CHA SUNG-HUI
Format Patent
LanguageEnglish
Published 06.05.1991
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Abstract A method for the production of anti T-2 toxin monoclonal antibody or its reactive fragment comprises (a) in vitro immunization of B-lymphocyte of rodents by antigen using T-2 toxin as hapten, (b) cell fusion of the immunized lymphocyte and myeloma cell to form hybridoma, (c) selecting for clones producing specific monoclonal antibodies to the antigen or hapten, (d) growing the selected clones by cell culture, and (e) collecting the produced monoclonal antidodies.
AbstractList A method for the production of anti T-2 toxin monoclonal antibody or its reactive fragment comprises (a) in vitro immunization of B-lymphocyte of rodents by antigen using T-2 toxin as hapten, (b) cell fusion of the immunized lymphocyte and myeloma cell to form hybridoma, (c) selecting for clones producing specific monoclonal antibodies to the antigen or hapten, (d) growing the selected clones by cell culture, and (e) collecting the produced monoclonal antidodies.
Author OH SANG-GON
CHA SUNG-HUI
KIM JION
CHONG YUN-SU
YUN HUI-JU
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Snippet A method for the production of anti T-2 toxin monoclonal antibody or its reactive fragment comprises (a) in vitro immunization of B-lymphocyte of rodents by...
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SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Title PROCESS MAKING OF ANTI T-2 TOXIN MONOCLONAL ANTIBODY AND METHOD ANALYSIS A T-2 TOXIN
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