세포의 동결보호 및 동결건조보호 방법

• Main Authors: KARDORFF MARKUS, JERE DHANANJAY
• Format: Patent
• Language: Korean
• Published: 29.02.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TRAPPING; VINEGAR; WINE

생존 가능한 세포를 유지하면서 냉동(freezing) 또는 동결건조(lyophilization)가 가능한 세포 집단을 포함하는 조성물이 제공된다. 상기 조성물은 단당류, 우레아(urea) 및 일부 실시양태에서는 증량제(bulking agent)를 포함한다. 또한, 세포를 제조하는 방법 및 저장 중에 세포가 생존 가능한 상태를 유지하도록 하는 방식으로 냉동 또는 동결건조를 통해 저장하는 방법이 제공된다. Provided are compositions comprising a population of cells that are amenable to freezing or lyophilization while maintaining viable cells. The compositions comprise a monosaccharide, urea and, in some embodiments, a bulking agent. Also provided are methods for preparing and storing cells via freezing or lyophilization in a manner that allows cells to remain viable during storage.