Detection of Target Nucleic Acid Sequences Using Different Detection Temperatures

• Main Authors: CHUN JONG YOON, LEE YOUNG JO
• Format: Patent
• Language: English; Korean
• Published: 02.02.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

본 발명은 상이한 검출 온도를 이용한 타겟 핵산 서열의 검출에 관한 것이다. 상이한 검출 온도를 이용하는 본 발명은 하나의 반응 용기에서 단일 유형의 표지만으로도 종래의 실시간 방식으로 복수의 타겟 핵산 서열을 검출할 수 있다. 종래 기술은 타겟 증폭 후 멜팅 분석을 통해 복수의 타겟 핵산 서열을 검출한다. 이와 달리, 본 발명은 타겟 증폭 후 멜팅 분석을 필요로 하지 않아, 분석 시간이 획기적으로 단축된다. The present invention relates to detection of target nucleic acid sequences using different detection temperatures. The present invention employing different detection temperatures enables to detect a plurality of target nucleic acid sequences in conventional real-time manners even with a single type of label in a single reaction vessel. The conventional technologies detect a plurality of target nucleic acid sequences by a melting analysis after target amplification. Unlikely, the present invention does not require a melting analysis after target amplification, such that the time for analysis is greatly reduced.