A Purification Method of Botulinum Toxin

The present invention relates to a method for purifying botulinum toxin comprising: (a) a step of preprocessing a culture solution containing the botulinum toxin; (b) a step of purifying the proprecessed botulinum toxin by using cation exchange chromatography; (c) a step of purifying the same by usi...

Full description

Saved in:
Bibliographic Details
Main Authors CHOI SEUNG KWAN, LIM HYUN TAEK, KIM JAE YOUNG, CHOI JIN HEE, NAM JEONG SUN, KIM SEUNGHO
Format Patent
LanguageEnglish
Korean
Published 23.10.2020
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
Online AccessGet full text

Cover

More Information
Summary:The present invention relates to a method for purifying botulinum toxin comprising: (a) a step of preprocessing a culture solution containing the botulinum toxin; (b) a step of purifying the proprecessed botulinum toxin by using cation exchange chromatography; (c) a step of purifying the same by using hydrophobic chromatography; and (d) a step of purifying the botulinum toxin by using anion exchange chromatography. The present invention is provided to purify the botulinum toxin with excellent purity and activity, thereby being usefully used for production of the botulinum toxin. 본 발명은 (a) 보툴리눔 독소를 포함하는 배양액을 전처리하는 단계; (b) 전처리된 보툴리눔 독소를 양이온 교환 크로마토그래피를 이용하여 정제하는 단계; (c) 소수성 크로마토그래피를 이용하여 정제하는 단계; 및 (d) 음이온 교환 크로마토그래피를 이용하여 보툴리눔 독소를 정제하는 단계를 포함하는 보툴리눔 독소의 정제방법에 관한 것이다. 본 발명에 따르면 순도 및 활성이 뛰어난 보툴리눔 독소를 정제할 수 있어, 보툴리눔 독소 생산에 유용하게 활용될 수 있다.
Bibliography:Application Number: KR20200044717