Freezing method of organoids

• Main Authors: YOO JONG MAN, CHOI JUNG KYU, HAN SOO JUNG
• Format: Patent
• Language: English; Korean
• Published: 15.10.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention provides an improved freezing method for long term preservation of organoids, including the steps of (a) leaving organoids at 4-10°C for at least 20 minutes in a medium containing fetal bovine serum and dimethyl sulfoxide; and (b) freezing the mixture obtained in the step (a)....