Freezing method of organoids

The present invention provides an improved freezing method for long term preservation of organoids, including the steps of (a) leaving organoids at 4-10°C for at least 20 minutes in a medium containing fetal bovine serum and dimethyl sulfoxide; and (b) freezing the mixture obtained in the step (a)....

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Bibliographic Details
Main Authors YOO JONG MAN, CHOI JUNG KYU, HAN SOO JUNG
Format Patent
LanguageEnglish
Korean
Published 15.10.2019
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:The present invention provides an improved freezing method for long term preservation of organoids, including the steps of (a) leaving organoids at 4-10°C for at least 20 minutes in a medium containing fetal bovine serum and dimethyl sulfoxide; and (b) freezing the mixture obtained in the step (a). 본 발명은(a) 오가노이드를 우태혈청 및 디메틸설폭시드를 포함하는 배지 중에서 20분 이상 4 ∼ 10 ℃에서 정치하는 단계; 및 (b) 단계(a)에서 얻어진 혼합물을 동결시키는 단계를 포함하는, 오가노이드(organoids)의 장기 보존을 위한 개선된 동결방법을 제공한다.
Bibliography:Application Number: KR20180039845