글리콜산 및/또는 글리옥실산의 생산을 위한 방법 및 미생물

• Main Authors: DISCHERT WANDA, CORRE GWENAELLE, SOUCAILLE PHILIPPE
• Format: Patent
• Language: Korean
• Published: 04.09.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

본 발명은 글리콜산 및/또는 글리옥실산의 생산에 유용한 방법 및 재조합 미생물에 관한 것이다. 본 발명의 방법은 본 발명의 변형된 미생물의 발효의 단지 하나의 단계, 또는 본 발명의 변형된 미생물의 발효의 하나의 단계 및 글리콜산 또는 글리옥실산의 생물학적 또는 화학적 전환의 하나의 단계를 수반하며, 이때 본 발명의 미생물은 포스포케톨라제 유전자를 과다발현한다. The present invention relates to methods and recombinant microorganisms useful for the production of glycolic acid and/or glyoxylic acid. The methods of the invention involve either only one step of fermentation of the modified microorganisms of the invention or one step of fermentation of the modified microorganisms of the invention and one step of either biological or chemical conversion of the glycolic acid or of the glyoxylic acid. Glycolic and glyoxylic acid are produced by phosphoketolase overexpression while attenuating at least one of aceB, glcB, gcl and eda. It is shown that deletion of ycdW and controlled induction of aceA leads to higher amount of glyoxylic acid which is produced at expense of glycolic acid.