HUMANIZATION OF RABBIT ANTIBODIES USING A UNIVERSAL ANTIBODY FRAMEWORK

• Main Authors: URECH DAVID, BORRAS LEONARDO
• Format: Patent
• Language: English; Korean
• Published: 06.08.2019
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

본 발명은 보편적인 항체 수용체 프레임워크 및 비인간 항체, 예를 들면 래빗 항체를 보편적인 항체 수용체 프레임워크를 사용하여 그래프팅하는 방법에 관한 것이다. 본 발명의 방법에 의해 생산된 항체는 다양한 진단 및 치료 방법에 유용하다. PROBLEM TO BE SOLVED: To provide humanization of rabbit antibodies using universal antibody framework.SOLUTION: The invention relates to a universal antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using the universal antibody acceptor framework. The antibodies produced by the method of the invention is useful for various diagnostic and therapeutic applications. The invention provides methods for grafting rabbit and other non-human CDRs, into the soluble and stable light chain and/or heavy chain human antibody framework sequences disclosed herein, thereby generating humanized antibodies with superior biophysical properties. In particular, immunobinders generated by the methods of the invention exhibit superior functional properties such as solubility and stability.