활성화된 포르밀글리신-생성 효소 및 그것의 생산 및 사용 방법

• Main Authors: RABUKA DAVID, DEHART GREGORY W, HOLDER PATRICK, BAKER JEANNE
• Format: Patent
• Language: Korean
• Published: 27.09.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

본 개시물은 활성화된 포르밀글리신-생성 효소 (FGE), 활성화된 FGE를 생산하는 방법, 및 포르밀글리신 (FGly) 잔기를 포함하는 단백질을 생산하는 방법에서 그것들의 사용을 제공한다. 활성화된 FGE의 생산 방법, 뿐만 아니라 FGly-함유 단백질의 생산에서 활성화된 FGE의 사용 방법은 세포-기반 및 무세포 방법 둘 다를 포함한다. 예를 들어, 본 개시물의 방법을 실시하는데 사용되는 조성물 및 키트가 또한 제공된다. The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.