MEANS AND METHODS FOR PRODUCING AMIDE COMPOUNDS WITH LESS ACRYLIC ACID

• Main Authors: BALDENIUS KAI UWE, OEDMAN PETER, BRAUN MICHAEL, BUDDE MICHAEL, KLEINER MATTHIAS, FREYER STEPHAN, DAEUWEL JUERGEN
• Format: Patent
• Language: English; Korean
• Published: 05.06.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

본 발명은 생체 촉매로서 니트릴 히드라타아제 (NH아제) 및 아미다아제 생성 미생물을 사용하여 부산물로서 적은 아크릴산을 갖는 니트릴 화합물로부터 아미드 화합물을 생성하는 수단 및 방법에 관한 것이다. 또한 본 발명의 방법에 의해 수득된 수성 아미드 화합물, 및 아크릴아미드 또는 폴리아크릴아미드를 포함하는 조성물 및 상기 니트릴 화합물을 아미드 화합물로 전환하기 위해 니트릴 화합물과 접촉될 때 적어도 400 의 NH아제/아미다아제 활성 비율을 나타내는 건조 미생물이 제공된다. The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.