Recombinant gene containing heat-labile enterotoxin B subunit and ApxIIA fragment, transgenic plant expressing LTB-ApxIIA fusion gene, and uses thereof

• Main Authors: KIM, TAE GEUM, YANG, MOON SIK
• Format: Patent
• Language: English; Korean
• Published: 12.06.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE: A recombinant plant expression vector having a fusion protein containing E.coli endotoxin B subunit(LTB) gene and an Actinobacillus pleuropneumoniae-derived ApxIIA gene fragment is provided to effectively manufacture a vaccine for pleuropneumonia. CONSTITUTION: A recombinant plant expression vector has a fusion gene containing E.coli-derived endotoxin B subunit(LTB) gene and Actinobacillus pleuropneumoniae-derived ApxIIa gene fragment. The ApxIIA gene fragment is ApxIIA619-801 gene having a base sequence of sequence number 1. A method for preparing the LTB-ApxIIA fusion protein comprises a step of transforming a plant with a vector and expressing the fusion gene. A vaccine composition for pleuropneumonia contains LTB-ApxIIA fusion protein as an active ingredient.