STRAIN DEVELOPEMENT AND PRODUCTION METHOD OF HUMAN LACTOFERRICIN PEPTIDE PRODUCTION STRAIN WHICH PRODUCES STRONG ANTI-BACTERIAL ACTIVITY

PURPOSE: A human-lactoferricin protein fragment-producing recombinant strain is provided to produce human-derived lactoferricin. CONSTITUTION: A human-lactoferricin peptide-producing recombinant strain has a system expressing or overexpressing latoffericin peptide into a strain using an expression s...

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Bibliographic Details
Main Authors LEE, HYUN HWAN, SONG, GA EUN, JO, JAE HYUNG
Format Patent
LanguageEnglish
Korean
Published 24.11.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: A human-lactoferricin protein fragment-producing recombinant strain is provided to produce human-derived lactoferricin. CONSTITUTION: A human-lactoferricin peptide-producing recombinant strain has a system expressing or overexpressing latoffericin peptide into a strain using an expression system. A recombinant vector is used as a fusion factor. The expression is induced by adding 0.1mM of IPTG under 20°C to induce normal expression or block inclusion body generation of MMIS fusion human lactoffericin peptide in culturing the recombinant strain. The recombinant strain is E.coli.
Bibliography:Application Number: KR20090042412