Discriminating method of Jindo-Hongju pigment

• Main Authors: PARK, KEUN HYUNG, KIM, SEONG HO, SEO, JI U, JANG, MI YEONG, MUN, YEONG HWAN, CHO, JEONG YONG
• Format: Patent
• Language: English; Korean
• Published: 10.06.2004
• Edition: 7
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

PURPOSE: A discriminating method of Jindo-Hongju pigment is provided, thereby easily and accurately discriminating whether red pigment of Jindo-Hongju is derived from puccoon (Lithospermum erythrorhizon Sieb. et Zucc), and maintaining constant quality of Jindo-Hongju pigment. CONSTITUTION: The discriminating method of Jindo-Hongju pigment comprises the steps of: mixing Jindo-Hongju pigment with ethylacetate-hexane mixed solvent in a same ratio, or mixing Jindo-Hongju pigment diluted with 10 to 20% of water with ethyl acetate in a same ratio, settling the mixture for a certain time, and observing color of an upper organic layer with the naked eye; determining whether the Jindo-Hongju pigment has a peak with the highest optical density value at 520 nm, and peaks with second and third optical density values at 496 nm and 550 nm using a spectrophotometer; and measuring the elution pattern by subjecting the Jindo-Hongju pigment to HPLC using the octadecylsilane column. 본 발명은 진도홍주색소의 감별 방법에 관한 것으로, 지초를 사용하여 제조된 진도홍주를 분액여두를 이용하여 에칠아세테이트계 용매계로 분배하면 진도홍주의 홍색소가 이들 유기용매계로 분배되어지는 성질을 이용하여 감별하는 1단계 감별방법, 분광광도계의 의한 진도홍주색소의 분광학적 성질을 이용하여 감별하는 2단계 감별방법, 그리고 옥타데실실란 컬럼이 장착된 고성능액체크로마토그라피에 의해 진도홍주 홍색소 용출양상을 검토하여 감별하는 3단계 감별방법으로 구성된 진도홍주색소의 1, 2, 3단계 감별방법에 의해 진도홍주 홍색소가 지초유래의 색소인지를 간편하고도 정확하게 식별할 수 있는 감별방법을 제공한다.