METHODS OF CULTURE, EXTRACTION AND ANALYSIS FOR PRODUCING ACTIVE ANTI-TUMOR SUBSTANCE, DECURSINOL ANGELATE, VIA A ROOT CULTURE OF ANGELICA GIGAS NAKAI

PURPOSE: Provided are methods of culture, extraction and analysis for producing an active anti-tumor substance, decursinol angelate, via a root culture of Angelica gigas Nakai, thereby stably mass-producing decursinol angelate in a short time, regardless of environmental conditions, such as weather,...

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Main Authors AHN, GYEONG SEOP, KIM, DONG IL, KIM, IK HWAN, KIM, MYEONG HWAN, CHO, JI SUK
Format Patent
LanguageEnglish
Korean
Published 16.01.2002
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: Provided are methods of culture, extraction and analysis for producing an active anti-tumor substance, decursinol angelate, via a root culture of Angelica gigas Nakai, thereby stably mass-producing decursinol angelate in a short time, regardless of environmental conditions, such as weather, soils, and damage from disease and harmful insects. CONSTITUTION: Angelica gigas Nakai. roots are aseptic-cultured in a flask containing 3-indolebutyric acid as a plant growth regulator and methyl jasmonate as an elicitor. The active anti-tumor substance, decursinol angelate, represented by the formula(1) is extracted by the steps of: 1) adding ethanol to the cultured root tissue and heating it at 50deg.C.; 2) evaporating ethanol from the resulting extract solution; 3) adding the same amount of chloroform to the resulting extract and extracting decursinol into an organic solvent layer; 4) obtaining the residue after evaporating chloroform. The decursinol angelate is analyzed by high performance liquid chromatography(HPLC) at 330nm using a reverse phase C-18 column.
Bibliography:Application Number: KR20000035530