REDOX MEDIATOR REAGENT AND BIOSENSOR

• Main Authors: GERBER, MARTIN T, BATESON, JOSEPH E, KOST, KENT M, OCHS, M. LUANN, WALLING, P. DOUGLAS, POLLMANN, KLAUS H
• Format: Patent
• Language: English; Korean
• Published: 18.02.1999
• Edition: 6
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

본 발명은 유체 샘플중의 분석물의 농도를 검출하기 위한 전류 적정 바이오센서를 제공한다. 바이오센서(1)는 절연재료97)에 부착된 작업전극(4) 및 상대전극(5)을 갖는다. 바이오센서 시약(11)은, 이것이 실제로 전극(4,5)의 노출된 표면(10)의 전부를 덮도록, 벽(9)에 위치한다. 시험을 위해, 분석물을 함유하는 샘플은 시약 벽(9)에 첨가되어, 반응이 완결되도록 하고, 전극(4,5)에 인가된 전위차 및 확산 제한 전류가 측정된다. A biosensor that includes working and counter electrodes of substantially the same size and made of the same electrically conducting material. A reagent is contained in a reagent well in the biosensor. The reagent includes a redox mediator, an enzyme, and a buffer, and covers substantially equal surface areas of portions of the working and counter electrodes. When a sample, containing the analyte to be measured, is added to the reagent well, a reaction occurs that oxidizes the analyte and reduces the redox mediator. After this reaction is complete, an electrical potential difference is applied between the electrodes. The amount of oxidized form of the redox mediator at the counter electrode and the applied potential difference must be sufficient to cause diffusion limited electrooxidation of the reduced form of the redox mediator at the surface of the working electrode. After a short time delay, current produced by the electrooxidation of the reduced form of the redox mediator is measured and the observed current is correlated to the amount of the analyte in the sample. In some cases, the analyte sought to be measured may be reduced and the redox mediator may be oxidized. In such cases, current produced by diffusion limited electroreduction of the oxidized form of the redox mediator is measured and correlated to the amount of analyte in the sample.