TISSUE CULTURE PLATE

• Main Authors: AKIYAMA JIRO, IMAI KOICHI, NOMURA TAKEO, YAMAMOTO TAKESHI, KAWAHARA HARUYUKI
• Format: Patent
• Language: English
• Published: 06.03.1987
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

PURPOSE:To eliminate the error in microscopic observation caused by the concave meniscus formed between the well wall and the culture liquid, by providing an inclined wall having a specific inclination angle at the top of the circumferential wall of a bottom-closed small chamber in a tissue-culture plate. CONSTITUTION:The objective tissue culture plate is composed of a bottom plate 10, a circumferential wall 11 attached nearly perpendicularly to the bottom wall, and an inclined wall 12 inclined outward from the top of the circumferential wall at an inclination angle of theta nearly equal to the contact angle between the well material and the culture liquid. The concave meniscus formed by placing the culture plate at rest is cancelled by the inclination angle theta of the inclined wall. The liquid surface becomes nearly flat, the disturbance in the observation field can be eliminated and the accuracy of measured cell density can be improved.