ZFY GENE AND ZFX GENE

• Main Authors: SUDOU SHIZUYO, SAI AKIRA
• Format: Patent
• Language: English
• Published: 18.11.1997
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; SUGARS; TRAPPING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain a Zfy gene cording for a polypeptide containing a specific amino acid sequence having a Zfy activity and a Zfx gene coding for a polypeptide containing a specific amino acid sequence having a Zfx activity, which are useful as primers, probes, markers, etc. SOLUTION: These genes are a new polynucleotide containing the all or a part of a base sequence coding for a polypeptide having a Zfy activity expressed by formula I or its complementary sequence and a new polynucleotide containing the all or a part of a base sequence coding for a polypeptide having a Zfx activity expressed by formula II or its complementary sequence. The both genes hybridize with a specific position of an X chromosome and a specific position of a Y chromosome, respectively, and are useful as primers, probes, markers, etc. The genes are obtained by collecting genome DNAs of a male of Tokudaia osimensis osimensis, preparing a genome library using the collected genome DNAs and screening the resultant genome library using a part of a Zfy2 gene of a mouse as a probe.