BOVINE Y CHROMOSOME SPECIFIC DNA AND ITS USE AS MARKER COMMON TO MALE AND FEMALE

• Main Authors: SAI AKIRA, SATO SEIJI, SUDOU SHIZUYO, KUDO TOSHIYUKI, NAKAMURA TOYORO
• Format: Patent
• Language: English
• Published: 08.10.1996
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

PURPOSE: To obtain a bovine Y chromosome specific DNA and/or a marker containing a DNA common to male and female useful for distinguishing a sex chromosome from an autosomal chromosome, an X CHR from a Y CHR and a short arm from a long arm in the Y CHR. CONSTITUTION: A female bovine DNA is isolated, a fragment having no regularity in DNA sequences at both ends is prepared from the DNA, is mixed with a DNA fragment obtained by treating a male bovine DNA with II type restriction enzyme and preparing regular ends and reassociated. Only a duplex DNA having regular ends is incorporated into a cloning vector having a restriction end contemporary with the ends, transduced into a host to give a DNA fragment having a male specific sequence, its base sequence is determined to give a sequence of the formula. The DNA fragment is used as a probe, a second and a third DNAs having male specific sequences are isolated from a male bovine DNA genome library, and a fourth DNA providing different association images between male and female and a fifth to a seventh DNAs having sequences common to male and female are obtained by Southern blot method.