DNA FRAGMENT HAVING PROMOTER FUNCTION

PURPOSE:To obtain the subject novel DNA fragment capable of strongly expressing a useful gene in a coryne type bacterium and capable of controlling the expression of the gene by changing the compositional condition of a culture medium, etc. CONSTITUTION:This DNA fragment is obtained from the chromos...

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Bibliographic Details
Main Authors YUGAWA HIDEAKI, TOOMASU JIEI ZUPANCHITSUKU
Format Patent
LanguageEnglish
Published 11.04.1995
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To obtain the subject novel DNA fragment capable of strongly expressing a useful gene in a coryne type bacterium and capable of controlling the expression of the gene by changing the compositional condition of a culture medium, etc. CONSTITUTION:This DNA fragment is obtained from the chromosome of a coryne type bacterium such as Brevibacterium flavum MJ-233, has a stronger function than of a tac promoter in the coryne tyep bacterium, and has e.g. a base sequence of the formula. The DNA fragment is obtained from the coryne bacterium with a vector used for the detection of plasmids and having (a) a plasmid-replicating and multiplying DNA region functioning in Escherichia coil, (b) a plasmid-replicating and multiplying DNA region functioning in the coryne type bacterium, (c) a DNA region containing a plasmid marker gene, (d) a DNA region containing a reporter gene, and (e) a transcription termination factor placed on the upstream of the (d) region.
Bibliography:Application Number: JP19940127816