SEPARATION AND PURIFICATION OF NEOTREHALOSE

• Main Authors: SHIODA MASAO, TAKAKU HAJIME, YATAKE TSUNEYA
• Format: Patent
• Language: English
• Published: 20.12.1994
• Edition: 5
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

PURPOSE:To obtain a neotrehalose by industrially advatageous separation and purification method characterized by simple operations using a column chromatography without decomposing intermixed other disaccharides, to say nothing of monosaccharides, trisaccharides and higher ones. CONSTITUTION:The method of separating and purifying a neotrehalose characterized by (l ) applying a mixture solution of the trehalose to a column chromatography consists of a stationary phase of a silica gel bonded with a long chain alkyl group and a moving phase of water or an aqueous solution of organic solvents, and (2) after applying the above solution to a column chromatography of a salt type strongly acidic cation exchange resin, applying the elute solution to the column chromatography which consists of a stationary phase of a silica gel bonded with a long chain alkyl group and a moving phase of water or an aqueous solution of organic solvents.