METHOD AND EQUIPMENT FOR MEASURING SOLID-PHASE BIOLOGICAL PECULIAR REACTION

• Main Authors: NISHIGUCHI HIROO, TANEBE MASARU, HANIYU TSUNEO
• Format: Patent
• Language: English
• Published: 10.11.1992
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

PURPOSE:To obtain a method for measuring a substance based on a biological peculiar reaction which has a high accuracy and achieves a stable analysis result. CONSTITUTION:In a solid-phase biological peculiar reaction measurement method, a sample containing a substance to be measured (A), a second substance which reacts peculiarly with the substance to be measured which is obtained by connecting signal generation substances to be detected or reacts with this first substance (B), and a washing liquid (C) are supplied in sequence from an upper portion of a porous matrix of a solid-phase biological peculiar reaction measuring equipment where a porous matrix in that a first substance which reacts with a substance to be measured peculiarly is connected to a filter which is constituted by a glass fiber whose average diameter ranges from 0.3 to 2.0mum is used as a solid phase and an absorption layer for absorbing a liquid solution which passes through the porous matrix is provided through or without through an inverse-flow prevention layer. An amount of substance to be measured remaining on the porous matrix is obtained by measuring a signal-generation substance.