METHOD FOR DETECTING ACCURATELY TWO BASES CONTINUOUS REPLACEMENT IN VARIANT SARS-CoV-2

• Main Authors: TERABAYASHI YASUNOBU, SHIMOKAWA AYAKA, SAGARA TAKEHIRO, SAITO KENSUKE, AKITOMO MIWA
• Format: Patent
• Language: English; Japanese
• Published: 09.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide a method for uniquely and easily detecting a SARS-CoV-2 in which variation occurs, since, a variant of the SARS-CoV-2 cannot be easily distinguished between itself and an original virus, in a conventional virus detection method, and although it is possible to detect comprehensively, variation, by interpreting a base sequence of a virus genome, the method requires a high speed sequencer and the method cannot be quickly and easily executed.SOLUTION: The inventors have found oligonucleotide which is useful for comparing genome RNA sequences of SARS-CoV-2 and a variant thereof, and examining them, and detecting a virus variant in which a specific variation occurs in a spike protein, by a nucleic acid amplification method. The inventors also have constructed a detection method of a variant SARS-CoV-2 using the oligonucleotide.SELECTED DRAWING: None 【課題】ＳＡＲＳ－ＣｏＶ－２の変異株は、これまでに構築されたウイルス検出方法では元のウイルスと区別することが難しかった。ウイルスゲノムの塩基配列を解読することにより変異を網羅的に検出することは可能であるが、高速シーケンサーを必要とするなど、迅速、簡便に実施可能な方法ではない。以上のとおり、変異が生じたＳＡＲＳ－ＣｏＶ－２を特異的にかつ簡便に検出できる方法が求められていた。【解決手段】ＳＡＲＳ－ＣｏＶ－２ならびにその変異株のゲノムＲＮＡ配列を比較、検討し、スパイクタンパク質に特定の変異が生じたウイルス変異株を核酸増幅法により検出するのに有用なオリゴヌクレオチドを見出した。さらに、当該オリゴヌクレオチドを使用する変異型ＳＡＲＳ－ＣｏＶ－２の検出方法を構築した。【選択図】 なし