CLOSED PERFUSION SYSTEM

• Main Author: GOTO HARUNORI
• Format: Patent
• Language: English; Japanese
• Published: 28.09.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

To provide a perfusion system that can automatically perform both medium exchange and continuous perfusion in a stable culture environment even in a closed system.SOLUTION: A waste liquid system is realized by operating a pump in the direction of suctioning the inside of a culture container, and opening a discharge path tube 11 for taking out a liquid in the container and an open path tube 15 using a pinch valve. At this time, since an airtight container is in a natural open state, outside air flows into the open path tube 15 through an air filter and is taken into the airtight container, and at the same time, the culture medium in the airtight container is collected into a waste liquid bottle through the discharge path tube 11 for taking out the liquid in the container. A liquid supply system is realized by operating the pump in the direction of sucking the inside of the culture container, and opening a liquid supply path tube 3 and a discharge path tube 7 for maintaining a liquid level, using the pinch valve. The culture container is an airtight container, the culture medium flowing into the liquid supply path tube 3 from a culture medium bottle is supplied into the airtight container, and at the same time, the air constituting the gas phase in the airtight container is collected into the waste liquid bottle through the discharge path tube 7 for maintaining the liquid level.SELECTED DRAWING: Figure 1 【課題】閉鎖系でも安定化した培養環境で培地交換と持続灌流を共に自動的に実施できる灌流システムの提供。【解決手段】廃液系は、ポンプが培養容器内を吸引する方向で動作し、ピンチバルブで容器内液取出し用排出路のチューブ１１、開放路のチューブ１５を開放させると実現される。このとき、密閉容器は自然開放状態になるので、エアフィルターを介して開放路のチューブ１５に外気が流入して密閉容器内に取入れられるのと並行して、密閉容器内の培地が容器内液取出し用排出路のチューブ１１を通って廃液瓶に回収される。液供給系は、ポンプが培養容器内を吸引する方向で動作し、ピンチバルブで液供給路のチューブ３、液面維持用排出路のチューブ７を開放させると実現される、培養容器は密閉容器になっており、培地瓶から液供給路のチューブ３に流入した培地が密閉容器内に供給されるのと並行して、密閉容器内の気相を構成する空気が液面維持用排出路のチューブ７を通って廃液瓶に回収される。【選択図】 図１