SINGLE-CELL WHOLE GENOME LIBRARIES FOR METHYLATION SEQUENCING

• Main Authors: DMITRY K POKHOLOK, RYAN MULQUEEN, STEVEN NORBERG, FRANK J STEEMERS, ANDREW C ADEY
• Format: Patent
• Language: English; Japanese
• Published: 20.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells.SOLUTION: The methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques in order to characterize methylation profiles of large...