SINGLE-CELL WHOLE GENOME LIBRARIES FOR METHYLATION SEQUENCING

• Main Authors: DMITRY K POKHOLOK, RYAN MULQUEEN, STEVEN NORBERG, FRANK J STEEMERS, ANDREW C ADEY
• Format: Patent
• Language: English; Japanese
• Published: 20.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells.SOLUTION: The methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques in order to characterize methylation profiles of large numbers of single cells quickly, accurately and inexpensively.SELECTED DRAWING: None 【課題】本明細書では、複数の単一細胞由来の核酸のメチル化状態を決定するための配列決定ライブラリーの調製方法が提供される。【解決手段】本方法は、多数の単一細胞のメチル化プロファイルを迅速に、正確に、且つ安価で特徴付けるために、スプリットアンドプール組み合わせインデックス付加と亜硫酸水素処理技法とを組み合わせる。【選択図】なし