DNA ENZYMES AND RNA CLEAVAGE METHODS

• Main Authors: INOMATA RIKA, MIYAGISHI MAKOTO
• Format: Patent
• Language: English; Japanese
• Published: 27.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide DNA enzymes capable of cleaving certain RNA, of which active center comprises a sufficiently small number of nucleotides.SOLUTION: Disclosed is a DNA enzyme having an activity to cleave a target RNA in the presence of zinc, where the enzyme comprises, from 5' to 3', a first arm region hybridizable with a region adjacent to 3' of cleavage region of the target RNA, a core region and a second arm region hybridizable with a region adjacent to 5' of the cleavage region of the target RNA, and the core region is a region of seven nucleotides or less containing a base sequence being 5'-AC-3', 5'-AA-3', 5'-AT-3', 5'-AG-3', 5'-GC-3', 5'-GA-3', 5'-GT-3', 5'-GG-3', 5'-ACA-3', 5'-TCA-3', 5'-GCA-3', 5'-GCT-3', or 5'-CCA-3'.SELECTED DRAWING: None 【課題】 活性中心の塩基数が十分に少ない、特定のＲＮＡを切断することが可能なＤＮＡ酵素を提供すること。【解決手段】 亜鉛存在下において標的ＲＮＡを切断する活性を有するＤＮＡ酵素であり、標的ＲＮＡの被切断領域の３'側に隣接する領域とハイブリダイズ可能な第１のアーム領域、コア領域、及び前記標的ＲＮＡの被切断領域の５'側に隣接する領域とハイブリダイズ可能な第２のアーム領域、を５'側からこの順で含み、かつ、前記コア領域が、５'−ＡＣ−３'、５'−ＡＡ−３'、５'−ＡＴ−３'、５'−ＡＧ−３'、５'−ＧＣ−３'、５'−ＧＡ−３'、５'−ＧＴ−３'、５'−ＧＧ−３'、５'−ＡＣＡ−３'、５'−ＴＣＡ−３'、５'−ＧＣＡ−３'、５'−ＧＣＴ−３'、又は５'−ＣＣＡ−３'である塩基配列を含む７塩基以下の領域である、ＤＮＡ酵素。【選択図】なし