CELL SUSPENSION PROCESSOR AND AUTOMATIC PASSAGE CULTURE SYSTEM USING THE SAME

• Main Authors: SHIMASE AKITA, ASO SADAMITSU, TAKADA EIICHIRO, IMAI KAZUNARI, SAKURAI TOMOYA, KAWARAI MASAKO
• Format: Patent
• Language: English; Japanese
• Published: 14.05.2020
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

To provide a cell suspension processor capable of achieving stable cell culture regardless of an operator's level of skill, and an automatic passage culture system using the same.SOLUTION: A cell suspension processor comprises: an inlet for taking in cell suspension; an outlet for discharging processed suspension; a channel which is provided between the inlet and the outlet and can hold the suspension; a liquid feeding pump; a cell dispersion degree/cell number density measurement device; a narrow portion; and a diluent container for diluting the suspension. The cell suspension processor further comprises a control unit which determines whether or not a dispersion degree of the cell has reached a prescribed dispersion degree on the basis of data obtained by the cell dispersion degree/cell number density measurement device, if the dispersion degree has not reached a prescribed dispersion degree, causes the cell suspension to pass through the narrow portion, and if the dispersion degree has reached the prescribed dispersion degree, determines a diluent amount for making a cell number density a desired density, and then drives the liquid feeding pump to take the amount of the diluent into the channel and mix the diluent with the cell suspension. An automatic passage culture system using the same is also provided.SELECTED DRAWING: Figure 9 【課題】作業者の熟練度に関係なく安定した細胞培養を実現する、細胞懸濁液処理装置、およびそれを用いた自動継代培養システムの提供。【解決手段】細胞懸濁液を取り込む入口、処理済みの該懸濁液を排出する出口、それらの間に設けられ該懸濁液を保持可能な流路、送液ポンプ、細胞分散度・細胞数濃度測定器、狭窄部、および該懸濁液を希釈する希釈液容器が備えられている細胞懸濁液処理装置であって、細胞分散度・細胞数濃度測定器により得たデータに基づいて、細胞が所定の分散度に達したか否かを判断し、所定の分散度に達していない場合には細胞懸濁液が前記狭窄部を通過するように、また達している場合には細胞数濃度を所望の濃度とするための希釈液量を判断し流路に取り込みかつ細胞懸濁液と混合するように送液ポンプを駆動させる制御部を有する細胞懸濁液処理装置、およびそれを用いた自動継代培養システム。【選択図】図９