FIBRONECTIN-BASED SCAFFOLD DOMAIN PROTEINS THAT BIND TO PCSK9

• Main Authors: DASA LIPOVSEK, PARKER REX A, BOWMAN MIAO, RAY CAMPHAUSEN, SHARON T CLOAD, DOREE F SITKOFF, AMNA SAEED-KOTHE, LOW CHEE MENG, TRACY S MITCHELL, CHING-HSIUNG FREDERICK LO, KATIE A RUSSO, DAVIS JONATHAN H, FABIENNE M DENHEZ, GINGER C RAKESTRAW
• Format: Patent
• Language: English; Japanese
• Published: 16.05.2019
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

To provide fibronectin-based scaffold domain proteins that bind to PCSK9.SOLUTION: Disclosed herein is a polypeptide comprising a fibronectin type III tenth domain (10Fn3), where the 10Fn3 comprises at least one loop selected from loops BC, DE and FG each having a modified amino acid sequence as compared to the corresponding loop of human 10Fn3 domain, and the polypeptide binds to PCSK9 (proprotein convertase subtilisin kexin type 9).SELECTED DRAWING: Figure 6 【課題】ＰＣＳＫ９に結合するフィブロネクチンベースのスカフォールドドメインタンパク質の提供。【解決手段】フィブロネクチンＩＩＩ型第１０ドメイン（１０Ｆｎ３）を含むポリペプチドであって、該１０Ｆｎ３は、ヒト１０Ｆｎ３ドメインの対応するループの配列と比較して、改変されたアミノ酸配列を有するループＢＣ、ＤＥおよびＦＧから選択される少なくとも１つのループを有し、該ポリペプチドは（前駆タンパク質転換酵素サブチリシンケキシン９型）ＰＣＳＫ９に結合するポリペプチド。【選択図】図６