NUCLEIC ACID AMPLIFICATION DEVICE

• Main Authors: NOGUCHI NORIMICHI, TACHIBANA HIROAKI
• Format: Patent
• Language: English; Japanese
• Published: 02.08.2018
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a nucleic acid amplification device that can efficiently amplify a target nucleic acid while being able to detect the initial concentration with one resolution.SOLUTION: A nucleic acid amplification device 1 comprises: an introduction part 11 for introducing a nucleic acid amplification reaction solution 100 containing a target nucleic acid 110; a discharge part 12 for discharging the nucleic acid amplification reaction solution 100; a flow path 13 for connecting the introduction part 11 and the discharge part 12; a plurality of microwells 14 formed in the flow path 13 for storing the nucleic acid amplification reaction solution 100; a first heating part 21 for heating the nucleic acid amplification reaction solution 100 stored in the plurality of microwells 14; and a second heating part 22 for heating a sealing fluid 200 introduced into the flow path 13 that seals the plurality of microwells 21.SELECTED DRAWING: Figure 3 【課題】標的核酸を効率良く増幅できるとともに初期濃度を１個の分解能で検出することができる核酸増幅装置を提供する。【解決手段】核酸増幅装置１は、標的核酸１１０を含む核酸増幅反応溶液１００を導入するための導入部１１と、核酸増幅反応溶液１００を排出するための排出部１２と、導入部１１と排出部１２とを結ぶ流路１３と、流路１３に形成され、核酸増幅反応溶液１００を格納する複数のマイクロウェル１４と、複数のマイクロウェル１４に格納された核酸増幅反応溶液１００を加熱する第１の加熱部２１と、複数のマイクロウェル２１を封止するために流路１３に導入される封止流体２００を加熱する第２の加熱部２２と、を備える。【選択図】図３