DETECTION OF NUCLEIC ACIDS

• Main Authors: TODD ALISON VELYIAN, DONALD CORTNY, LIT YEEN TAN, MOKANY ELISA, SAMANTHA WALKER, LONERGAN DINA
• Format: Patent
• Language: English; Japanese
• Published: 17.05.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide methods for determining the presence or absence of target nucleotides in samples.SOLUTION: A method of the invention comprises the steps of: providing a primer oligonucleotide comprising a first primer component terminating at the 5' end of the oligonucleotide, and capable of hybridizing to a first portion of a strand of the target polynucleotide by complementary base pairing, and a second primer component terminating at the 3' end of the oligonucleotide and capable of hybridizing to a second portion of a strand of the target polynucleotide by complementary base pairing; contacting a sample that may contain a target polynucleotide with the primer oligonucleotide under conditions suitable for hybridization between the first and second primer components thereby to form a double stranded duplex; and contacting the sample with a polymerase enzyme capable of using the target polynucleotide strand as a template.SELECTED DRAWING: Figure 1 【課題】試料中の標的ポリヌクレオチドの存在または不存在を決定する方法の提供。【解決手段】オリゴヌクレオチドの５'末端で終止し、標的ポリヌクレオチドの鎖の第１部分に相補的塩基対合によりハイブリダイズ可能な第１プライマー成分と、オリゴヌクレオチドの３'末端で終止し、標的ポリヌクレオチドの鎖の第２部分に相補的塩基対合によりハイブリダイズ可能な第２プライマー成分を含むプライマーオリゴヌクレオチドを準備すること、標的ポリヌクレオチドを含んでいる可能性のある試料を、第１プライマー成分と第２プライマー成分を標的ポリヌクレオチド鎖とハイブリダイズさせるのに適当な条件下で、プライマーオリゴヌクレオチドと接触させることであって、そうすることで２本鎖デュプレックスが形成され、試料を標的ポリヌクレオチド鎖を鋳型として用いることができるポリメラーゼ酵素と接触させることを含む方法。【選択図】図１