HIGH FIDELITY RESTRICTION ENDONUCLEASE

• Main Authors: XU SHUANG-YONG, GUAN SHENGXI, SUN DAPENG, HUANG YISHU, ZHU ZHENYU, ZHANG CHUNHUA, LAI XUHUI, AINE QUIMBY, LI XIANGHUI, CHAN SIU-HONG
• Format: Patent
• Language: English; Japanese
• Published: 18.01.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method and composition provided for engineering a mutant enzyme with reduced star activity where the mutant enzyme has a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.SOLUTION: According to the pr...